Inducing re-epithelialization in skin wound through cultured oral mucosal keratinocytes
김현실, 김남희, 김진, 차인호,
소속 상세정보
김현실 ( Kim Hyun-Sil ) - Yonsei University College of Dentistry Department of Oral Pathology
김남희 ( Kim Nam-Hee ) - Yonsei University College of Dentistry Oral Cancer Research Institute
김진 ( Kim Jin ) - Yonsei University College of Dentistry Oral Cancer Research Institute
차인호 ( Cha In-Ho ) - Yonsei University College of Dentistry Department of Oral and Maxillofacial Surgery
KMID : 0355620130390020063
Abstract
Objectives: The purpose of this study was to investigate the wound healing effect of primary cultured oral mucosal keratinocytes (OMKs) and to assess their roles in skin wounds.
Materials and Methods: OMK labeled with BromodeoxyUridine were scattered onto 1.5×1.5 cm skin defects of adult female nude mice (OMK group, n=15). For the control, culture media were placed on the wound (control group, n=15). Mice in both groups were sacrificed at three days (n=5), one week (n=5), and two weeks (n=5), and histomorphometric and immunoblot analyses with keratinocyte growth factor (KGF), interleukin (IL)-6, and IL-1? antibody were performed for the biopsied wound specimen. To verify the effect of the cytokine, rhIL-1? was applied instead of OMK transplantation, and the OMK and control groups were compared with regard to re-epithelialization.
Results: Histomorphometric analyses demonstrated faster re-epithelialization in the graft group than in the control group at the third day, first week, and second week. Newly forming epithelium showed maintenance of the histological character of the skin epithelium. The graft group showed superior expression of KGF, IL-6, and IL-1? protein, compared with the control group. Similar faster re-epithelialization was observed after treatment with rhIL-1? instead of OMK transplantation.
Conclusion: We successfully confirmed that the graft of primary cultured OMKs promoted regeneration of skin defects. The mechanism of accelerated wound healing by primary cultured OMKs was attributed to inducement of cytokine expression as required for re-epithelialization.
키워드
Oral mucosal keratinocyte; Primary cell culture; Wound healing; Tissue engineering; Cytokine
원문 및 링크아웃 정보
등재저널 정보